I1907
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 | Immunoblotting Whole cell extracts of Rat-1 (Lane A), NIH3T3 (Lane B), chicken fibroblasts (Lane C), HeLa (Lane D) and HUVEC (Lane E) cells was separated on SDS-PAGE, blotted with Rabbit Anti-ILK (Cat No. I1907) and developed using Goat anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. |
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I1907
Sigma
Anti-ILK antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| Synonym: | Anti-β1-Integrin-Linked Kinase |
| MDL number: | MFCD01633545 |
Description
| Immunogen | synthetic peptide corresponding to the C-terminus of human ILK (amino acids 435-452). The peptide sequence is identical in mouse, guinea pig, and chicken ILK-1 and is highly conserved (single amino acid substitution) in human ILK-2 and Drosophila ILK (>80% homology). |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 50 kDa |
| species reactivity | human, chicken, mouse |
| application(s) | immunoblotting: 1:4,000 using whole cell extract of chicken fibroblasts and human epitheloid carcinoma HeLa cells |
| indirect immunofluorescence: 1:100 using rat fibroblasts Rat-1 and mouse fibroblasts NIH3T3 cell lines | |
| microarray: suitable | |
| Gene Information | human ... ILK(3611) |
| mouse ... Ilk(16202) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
